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pcdna3 1 þ circrna mini vector  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 1 þ circrna mini vector
    Pcdna3 1 þ Circrna Mini Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+%C3%BE+circrna+mini+vector/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/pm35176309-360-14-20
    Average 96 stars, based on 247 article reviews
    pcdna3 1 þ circrna mini vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Over Expression:

    Article Title: Circular RNA circ_0001649 acts as a prognostic biomarker and inhibits NSCLC progression via sponging miR-331-3p and miR-338-5p.
    Article Snippet: Accumulating evidence documented the key functions of circular RNAs (circRNAs) in various malignancies.. However, the study regarding the involvement of circRNAs in non-small cell lung cancer (NSCLC) has just begun.. In the present study, qRT-PCR was used to determine the expression of circ_0001649 in NSCLC tissues and cells.

    Sequencing:

    Article Title: Circular RNA circ_0001649 acts as a prognostic biomarker and inhibits NSCLC progression via sponging miR-331-3p and miR-338-5p.
    Article Snippet: Accumulating evidence documented the key functions of circular RNAs (circRNAs) in various malignancies.. However, the study regarding the involvement of circRNAs in non-small cell lung cancer (NSCLC) has just begun.. In the present study, qRT-PCR was used to determine the expression of circ_0001649 in NSCLC tissues and cells.

    Article Title: Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
    Article Snippet: .. The human circANAPC7 sequence was amplified from human ANAPC7 transcript and then inserted into pcDNA3.1 (þ) circRNA mini vector (#60648; Addgene) using the EcoRV and SacII sites. circANAPC7 sequence and 30UTR and PHLPP2 were inserted into Dual-Luciferase miRNA Target Expression Vector pmiRGlo (#78132; Addgene) using the NheI and XbaI sites. .. The mutated vectors were generated by the QuikChange Lightning Site-Directed Mutagenesis Kit (#210518; Agilent Technologies).

    Synthesized:

    Article Title: Circular RNA circ_0001649 acts as a prognostic biomarker and inhibits NSCLC progression via sponging miR-331-3p and miR-338-5p.
    Article Snippet: Accumulating evidence documented the key functions of circular RNAs (circRNAs) in various malignancies.. However, the study regarding the involvement of circRNAs in non-small cell lung cancer (NSCLC) has just begun.. In the present study, qRT-PCR was used to determine the expression of circ_0001649 in NSCLC tissues and cells.

    Article Title: CircCDYL inhibits the expression of C-MYC to suppress cell growth and migration in bladder cancer.
    Article Snippet: .. CircRNA plasmids construction and transfection To construct circCDYL over-expression plasmids, human circCDYL cDNA was synthesized by TSINGKE (China) and cloned into pcDNA3.1 (þ) CircRNA Mini vector (Addgene, USA). .. The plasmids were transfected into cells lines by using Lipofectamine2000 (Life Technologies, USA) followed the manufacturer’s instructions.

    Clone Assay:

    Article Title: Circular RNA circ_0001649 acts as a prognostic biomarker and inhibits NSCLC progression via sponging miR-331-3p and miR-338-5p.
    Article Snippet: Accumulating evidence documented the key functions of circular RNAs (circRNAs) in various malignancies.. However, the study regarding the involvement of circRNAs in non-small cell lung cancer (NSCLC) has just begun.. In the present study, qRT-PCR was used to determine the expression of circ_0001649 in NSCLC tissues and cells.

    Article Title: CircCDYL inhibits the expression of C-MYC to suppress cell growth and migration in bladder cancer.
    Article Snippet: .. CircRNA plasmids construction and transfection To construct circCDYL over-expression plasmids, human circCDYL cDNA was synthesized by TSINGKE (China) and cloned into pcDNA3.1 (þ) CircRNA Mini vector (Addgene, USA). .. The plasmids were transfected into cells lines by using Lipofectamine2000 (Life Technologies, USA) followed the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Circular RNA circ_0001649 acts as a prognostic biomarker and inhibits NSCLC progression via sponging miR-331-3p and miR-338-5p.
    Article Snippet: Accumulating evidence documented the key functions of circular RNAs (circRNAs) in various malignancies.. However, the study regarding the involvement of circRNAs in non-small cell lung cancer (NSCLC) has just begun.. In the present study, qRT-PCR was used to determine the expression of circ_0001649 in NSCLC tissues and cells.

    Article Title: Circular RNA circZNF652 is overexpressed in osteoarthritis and positively regulates LPS-induced apoptosis of chondrocytes by upregulating PTEN.
    Article Snippet: .. The expression vectors of circZNF652 and PTEN were constructed with pcDNA3.1 vector (Invitrogen) and pcDNA3.1(þ) CircRNA Mini Vector (Addgene) as the backbone, respectively. .. SiRNA negative control (NC) and circZNF652 siRNA were purchased from Invitrogen.

    Article Title: Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
    Article Snippet: .. The human circANAPC7 sequence was amplified from human ANAPC7 transcript and then inserted into pcDNA3.1 (þ) circRNA mini vector (#60648; Addgene) using the EcoRV and SacII sites. circANAPC7 sequence and 30UTR and PHLPP2 were inserted into Dual-Luciferase miRNA Target Expression Vector pmiRGlo (#78132; Addgene) using the NheI and XbaI sites. .. The mutated vectors were generated by the QuikChange Lightning Site-Directed Mutagenesis Kit (#210518; Agilent Technologies).

    Produced:

    Article Title: Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
    Article Snippet: .. DNA spike-in was produced from the multiple cloning sites in pcDNA3.1(þ) CircRNA Mini Vector (#60648; Addgene). .. Transcription was blocked by adding 2 mg/mL actinomycin D (A1410; Sigma-Aldrich) or dimethyl sulfoxide control to the cell culture medium and cultured for 0, 4, 8, 12, or 24 hours.

    Cloning:

    Article Title: Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
    Article Snippet: .. DNA spike-in was produced from the multiple cloning sites in pcDNA3.1(þ) CircRNA Mini Vector (#60648; Addgene). .. Transcription was blocked by adding 2 mg/mL actinomycin D (A1410; Sigma-Aldrich) or dimethyl sulfoxide control to the cell culture medium and cultured for 0, 4, 8, 12, or 24 hours.

    Expressing:

    Article Title: Circular RNA circZNF652 is overexpressed in osteoarthritis and positively regulates LPS-induced apoptosis of chondrocytes by upregulating PTEN.
    Article Snippet: .. The expression vectors of circZNF652 and PTEN were constructed with pcDNA3.1 vector (Invitrogen) and pcDNA3.1(þ) CircRNA Mini Vector (Addgene) as the backbone, respectively. .. SiRNA negative control (NC) and circZNF652 siRNA were purchased from Invitrogen.

    Article Title: Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
    Article Snippet: .. The human circANAPC7 sequence was amplified from human ANAPC7 transcript and then inserted into pcDNA3.1 (þ) circRNA mini vector (#60648; Addgene) using the EcoRV and SacII sites. circANAPC7 sequence and 30UTR and PHLPP2 were inserted into Dual-Luciferase miRNA Target Expression Vector pmiRGlo (#78132; Addgene) using the NheI and XbaI sites. .. The mutated vectors were generated by the QuikChange Lightning Site-Directed Mutagenesis Kit (#210518; Agilent Technologies).

    Construct:

    Article Title: Circular RNA circZNF652 is overexpressed in osteoarthritis and positively regulates LPS-induced apoptosis of chondrocytes by upregulating PTEN.
    Article Snippet: .. The expression vectors of circZNF652 and PTEN were constructed with pcDNA3.1 vector (Invitrogen) and pcDNA3.1(þ) CircRNA Mini Vector (Addgene) as the backbone, respectively. .. SiRNA negative control (NC) and circZNF652 siRNA were purchased from Invitrogen.

    Article Title: CircCDYL inhibits the expression of C-MYC to suppress cell growth and migration in bladder cancer.
    Article Snippet: .. CircRNA plasmids construction and transfection To construct circCDYL over-expression plasmids, human circCDYL cDNA was synthesized by TSINGKE (China) and cloned into pcDNA3.1 (þ) CircRNA Mini vector (Addgene, USA). .. The plasmids were transfected into cells lines by using Lipofectamine2000 (Life Technologies, USA) followed the manufacturer’s instructions.

    Transfection:

    Article Title: CircCDYL inhibits the expression of C-MYC to suppress cell growth and migration in bladder cancer.
    Article Snippet: .. CircRNA plasmids construction and transfection To construct circCDYL over-expression plasmids, human circCDYL cDNA was synthesized by TSINGKE (China) and cloned into pcDNA3.1 (þ) CircRNA Mini vector (Addgene, USA). .. The plasmids were transfected into cells lines by using Lipofectamine2000 (Life Technologies, USA) followed the manufacturer’s instructions.

    Amplification:

    Article Title: Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
    Article Snippet: .. The human circANAPC7 sequence was amplified from human ANAPC7 transcript and then inserted into pcDNA3.1 (þ) circRNA mini vector (#60648; Addgene) using the EcoRV and SacII sites. circANAPC7 sequence and 30UTR and PHLPP2 were inserted into Dual-Luciferase miRNA Target Expression Vector pmiRGlo (#78132; Addgene) using the NheI and XbaI sites. .. The mutated vectors were generated by the QuikChange Lightning Site-Directed Mutagenesis Kit (#210518; Agilent Technologies).



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